Beckman-Coulter Epic XL

Description

• 3-color fluorescence with 4-color option
• Air-cooled 488nm 15mw laser
• 10u x 90u light beam
• Detector Range: 300nm-800nm
• Flow-Cell Size: 250u x 250u BioSense
• Max analysis rate: >3300 cells/sec
• Min Cell Size, um: <0.5
• Max analysis rate: >3300 cells/sec
• Color dot density; contour, isometric, tomogram, overlay, legend and projection plots. PRISM displays; color precedence or color-blend gating; automatic Boolean logic gate creation; 32 gates per file, real-time statistics.
• Intel Pentium processor and 4 network transputers for digital signal processing.
• Signal-intensity display, biohazard-safe triple-fluid (sheath/cleanse/waste) system with automated functions and variable flow rate; auto setup; SYSTEM II software with acquisition, list mode, multigraph, reports and utilities. Features FastSet and CellTracker. Opt tetraONE SYSTEM software automates 4-color acquisition and analysis. Retic-ONE SYSTEM software for reticulocyte enumeration. XL-MCL incorporates automated multitube carousel loader–directly compatible with CellPrep, PrepPlus and TQ-Prep sample preparation systems for approx. 100 tube/hr. throughput plus integrated bar-code scanner for 3-way sample ID (carousel, tube, position and sample tube).

The Beckman Coulter Epics XL Flow Cytometer system features the capability to analyze up to 4 colors of immunofluorescence from a single air cooled laser. Other multi-color applications include: multiparametric DNA analysis, platelet studies, reticulocyte enumeration, cell biology/functional studies and as well as a broad range of research applications. The instrument is self contained and biohazard safe. The user retains the flexibility to change filter elements for versatility in research settings. For high volume users, the XL-MCL system offers walk-away sample handling with the Multi Carousel Loader (MCL). Capable of throughputs of up to 100 samples per hour, the fully featured XL-MCL incorporates positive bar-code identification and true vortex mixing prior to aspiration. The XL remains the only flow cytometer to offer state-of-the-art Digital Signal Processing (DSP) for reliable linearity and drift-free amplification and compensation. The single laser design eliminates concerns regarding multi-beam stability, signal delay and alignment. Compensation is achieved by a unique digital compensation matrix.

The EXPO32 ADC software provides both acquisition and analysis options. This comprehensive software package gives you the power to simplify reporting, speed up data acquisition and analysis, track instrument use and get the most out of your data.

The COULTER EPICS XL-MCL is a laser-based flow cytometer which utilizes color-differentiated fluorescence and light scatter measurements to analyze cells. Cells in liquid suspension are presented under pressure to a flow cell where they are surrounded by a laminar sheath of particle-free liquid. This coaxial stream passes through a flow chamber as a jet. Cells are presented one at a time to a laser beam generated by a 488 nm argon laser. The instrument can handle up to 10,000 cells per second. As the cells pass through the laser beam they will (1) scatter light and/or (2) fluoresce, due to either autofluorescence or to the presence of fluorescent stains which have been selectively absorbed or bound by the cell. The scatter and fluorescence emissions are collected by detectors (photo diodes and photomultiplier tubes) which convert the signals to voltage pulses that are proportional to the amount of light scattered and/or to the intensity of the fluorescence. These pulses are then amplified and converted to a digital form which can be displayed in numeric and/or histogram formats. This instrument has four PMT?s allowing an investigator to look at four colors of fluorescence at once.

For high volume users, the XL-MCL system offers walk away sample handling for superior efficiency with the Multi Carousel Loader (MCL). Capable of throughputs of up to 100 samples per hour, the fully featured XL-MCL incorporates positive bar-code identification and true vortex mixing prior to aspiration.

The XL remains the only flow cytometer to offer state-of-the-art Digital Signal Processing (DSP) for reliable linearity and drift-free amplification and compensation. The single laser design eliminates concerns regarding multi-beam stability, signal delay and alignment. Compensation is achieved by a unique digital compensation matrix.

Weight: 140 Lbs.

Dimensions: 24(W) x 23(D) x 20(H) inches