Description
By dramatically improving your data quality, significantly reducing your cost per sample, and enabling you to perform more runs per day, the fully automated 48-capillary 3730 system gives your laboratory a tremendous technological and financial advantage. Which means you can pursue an exciting diversity of projects (and you can always upgrade to 96 capillaries when you need to).
Streamline Your Workflow
The 3730 DNA Analyzer system is engineered for highly reliable, unattended operation of up to 48 hours (applies to modules with run times longer than 30 minutes). Automation features which minimize the need for operator intervention and decrease the risk of human error include an integrated plate stacker, internal bar code reader, and onboard polymer delivery system.
Increase Productivity
The high signal-to-noise ratio ensures you get high-quality data — even when you use low-concentration samples and reagents. Labor-saving automation features minimize hands-on time and enable you to analyze more data more efficiently.
Get the Highest-Quality Sequencing and Genotyping Data
The enhanced optical design provides a higher signal-to-noise ratio and a more uniform signal profile across the array. This design, combined with our new, advanced polymer, enables the longest read lengths of any available system, and provides enhanced color balance for streamlined genotyping sample handling. In addition, exceptional sensitivity enables higher success rates across a wider range of sample templates and concentrations than other currently available systems.
One Instrument, Multiple Applications
With the 3730 DNA Analyzer, you get the highest-quality data from a wide array of applications. In addition to being ideal for high-throughput sequencing, the analyzer’s optimized application assays, instrument, and analysis software also provide a complete solution for genotyping and resequencing.
Integrated Data Analysis Tools Reduce Time-to-Results
The 3730 DNA Analyzer software suite allows you to generate more meaningful data with less work.
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System Setup Specifications
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Laser
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Argon-ion multi-line, single mode laser: primary excitation lines 488 and 514.5 nm
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Oven Temperature
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Active temperature control between 18°C to 70°C
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Integrated Plate Stacker
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Houses 16 sample plates at any time
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Sample Volumes
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For 384-Well Sample Plates: 5-30 µL, For 96-Well Sample Plates: 10-50 µL
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Plate Seal
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Septa or Polypropylene heat seal (automatic onboard piercing)
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Operating Environment
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Ambient temperature: 18°C to 30°C, Humidity: 20 to 80% (non-condensing)
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Capillary Array Lengths
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36cm or 50cm
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Capillary Dimensions
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150 5m o.d./50 5m i.d
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Polymer Consumption
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36cm: approximately 100 µL per run, 50cm: approximately 125 µL per run
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Performance/Throughput
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Performance by run module (Data Collection v2.0)
XLRseq:LongSeq: StdSeq: FastSeq: RapidSeq: GeneMapper® Software: |
50cm, 900 QV20 base/360 min 50cm, 850 QV20 base/120 min 36cm, 700 QV20 base/60 min 50cm, 700 QV20 base/60 min 36cm, 550 QV20 bases/36 min 36cm, up to 500 bp res w/ 0.15 bp precision in 34 min |
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Throughput by run module (Data Collection v2.0)
XLRseq:LongSeq: StdSeq: FastSeq: RapidSeq: GeneMapper® Software: |
384 samples/day (345 Kbases) 576 samples/day (490 Kbases) 1152 samples/day (775 Kbases) 1152 samples/day (800 Kbases) 1920 samples/day (1050 Kbases) 2112 samples/day (46,000 genotypes) |
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Physical Specifications
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Width
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100 cm, Computer: 18cm
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Depth
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73 cm, Computer: 45cm
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Weight
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approximately 180 kg, Computer: approx. 30kg
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Height
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89 cm, Computer: 43cm
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Applied Biosystems 3730 DNA Analyzer
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Optics
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Temperature control
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Software
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Plate Handling
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Capillaries
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Reagent Handling
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(Applies to run mod. w/ run times > 30 min)
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Computer
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Computer Specifications (PC)
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Operating System Compatibility
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Windows® 2000 Professional Edition, Windows® XP
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Memory
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1 GB400MHz RAM
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System Requirements
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Network Interface: Integrated 10/100/1000 NIC and secondary NIC (provided separately)*Windows is a registered trademark of Microsoft Corporation.
*Pentium is a registered trademark of Intel Corporation. |
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CD-ROM
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CDRW/DVD-ROM Combo Drive
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Floppy
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Floppy Drive
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Display
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17
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Hard Disk
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1st Drive-160 GB, 2nd Drive-250 GB (on GX280 Computer)
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CPU
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Pentium® IV 3.0 GHz Processor
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Power Requirements
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Voltage
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200-220 V or 230-240 V+/-10%
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Power Dissipated (W)
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2,000 watts (approximation)
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Frequency Hz
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50/60 Hz
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Circuit Current
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Maximum of 15 amps. Requires a 30 amp receptacle to match one of the two power cord configurations that ship with the system. The electrical receptacle must be within 3 m (10 ft.) of the back of the instrument. Do not use extension cords.
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Weight: 397 Lbs.
Dimensions: 39(W) x 29(D) x 35(H) inches
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